DigiM's work on the yeast cell sets the record for whole cell imaging with its 3 nanometer 3D spatial resolution in 2012. Published by Journal of BioTechniques, the article referenced below was spotlighted by the editorial board as the cover story. It later won the paper of the year award.

FIB-SEM Images and 3D Reconstruction of a Full Yeast Cell Using Three Nanometer Spatial Resolution from DigiM Solution on Vimeo.

We developed an approach for focused gallium-ion beam scanning electron microscopy with energy filtered detection of back-scattered electrons to create near isometric voxels for high resolution whole cell visualization. Specifically, this method allowed us to create three-dimensional volumes of high-pressure frozen, freeze-substituted Saccharomyces cerevisiae yeast cells with pixel resolutions down to 3 nm/pixel in x, y and z, supported by both empirical data and Monte Carlo simulations. As a result, we were able to segment and quantify datasets of numerous targeted sub-cellular structures/organelles at high-resolution, including the volume, volume percentage and surface area of the endoplasmic reticulum, cell wall, vacuoles, vacuoles, and mitochondria from an entire cell.

3D Visualization of Golgi and Vesicles Inside a Yeast Cell from DigiM Solution on Vimeo.

The ability to visualize, segment and quantify entire eukaryotic cells at a high-resolution will provide new perspectives and insights of the inner workings of cells.