FIB-SEM typically runs with a resolution of 3nm – 50nm. Assuming an image produced has 2000x2000 pixels along X and Y directions, its field of view might be about 6 – 100 micrometers in size.
This size is very small compared to most of the analyzed samples. Hence it is critical to make sure that the imaged volumes are representative.
Sample representativeness is strongly dependent on specific applications. Due to increasing microstructure heterogeneity in modern science, it is almost impossible to claim that one sampling strategy can fit all. To ensure sucessful sampling strategy, DigiM’s systematic approach has two important elements,